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DOTAP chloride

Name: DOTAP chloride
Brand: TargetMol
SKU: T11086
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11086Cas No. 132172-61-3

Alias 1,2-Dioleoyl-3-trimethylammonium-propane chloride

DOTAP chloride (1, 2-dioleoyl-3-trimethylammonium-propane chloride) is an efficient cationic lipid, and its target is mainly the negatively charged components on the cell membrane. DOTAP chloride binds to negatively charged nucleic acids (such as DNA and RNA) by electrostatic interaction to form complexes, thereby achieving the delivery of nucleic acids.

DOTAP chloride

🥰Excellent

Purity: 99.97%

Catalog No. T11086Alias 1,2-Dioleoyl-3-trimethylammonium-propane chlorideCas No. 132172-61-3

Pack Size	Price	Availability
25 mg	$31	In Stock
50 mg	$44	In Stock
100 mg	$81	In Stock
1 mL x 10 mM (in DMSO)	$48	In Stock

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Purity:99.97%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	DOTAP chloride (1, 2-dioleoyl-3-trimethylammonium-propane chloride) is an efficient cationic lipid, and its target is mainly the negatively charged components on the cell membrane. DOTAP chloride binds to negatively charged nucleic acids (such as DNA and RNA) by electrostatic interaction to form complexes, thereby achieving the delivery of nucleic acids.
Targets&IC50	293T cells:58.6 µM
In vitro	METHODS: Prostate cancer cell line PC3 and melanoma cell line M21 were treated with paclitaxel (PTX) loaded on DOTAP/DOPC liposome carriers (CLs) for 24 hours. The cytotoxicity was detected by measuring the absorbance at 490 nm using an enzyme-linked immunosorbent assay reader. RESULTS: With the increase of PTX content, the cytotoxic effect of DOTAP/DOPC CLs is enhanced. [1]
Synonyms	1,2-Dioleoyl-3-trimethylammonium-propane chloride

Chemical Properties

Molecular Weight	698.54
Formula	C₄₂H₈₀ClNO₄
Cas No.	132172-61-3
Smiles	[Cl-].CCCCCCCC\C=C\CCCCCCCC(=O)OC[C@H](C[N+](C)(C)C)OC(=O)CCCCCCC\C=C\CCCCCCCC
Relative Density.	no data available
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 130 mg/mL (186.1 mM), Sonication and heating are recommended.

DMSO: 125 mg/mL (178.94 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (5.73 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	1.4316 mL	7.1578 mL	14.3156 mL	71.5779 mL
5 mM	0.2863 mL	1.4316 mL	2.8631 mL	14.3156 mL
10 mM	0.1432 mL	0.7158 mL	1.4316 mL	7.1578 mL
20 mM	0.0716 mL	0.3579 mL	0.7158 mL	3.5789 mL
50 mM	0.0286 mL	0.1432 mL	0.2863 mL	1.4316 mL
100 mM	0.0143 mL	0.0716 mL	0.1432 mL	0.7158 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc